Rotor Gene 6000 Software Programs

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by liasisime1971 2020. 3. 4. 11:40

I figured I could deal with problems I was having earlier with rainbows at the beginning of my reaction.Speaking of which. I was able to determine that it was the tubes that were causing those early cycle anomalies. I figured I could deal with problems I was having earlier with rainbows at the beginning of my reaction.Speaking of which. I was able to determine that it was the tubes that were causing those early cycle anomalies.

I contacted the company that makes the tubes and they don't really have any answer as to why or how.Maybe a little late but:You could see att your raw-data that these rainbow curves are not a correct positive one.If your curve goes down in the beginning and then up after a while. The instrument can interpret it as a log curve.You have a function that can disregard raw-data that not goes over X% over NTC, say that you put it to 5% all of you rainbow curves will probebly go away, surely at 10%.

Read the manual about it and find out how it workes. It just a tool to use and you will not miss any 'correct' curves.

Rotor Gene 6000 Real-Time PCR Machine Features:. Unique centrifugal rotary format. Uniformity: ± 0.01°C. Resolution: 0.02°C (smallest programmable and reported thermal increment). 5-Plex (Green, Yellow, Orange, Red, Crimson) fluorescence channels. High resolution melt (HRM) application.

Holds 36 x0.2ml tubes, 72 x 0.1ml strip tubes or 100 Gene-Disc. Software: Build-in extensive analysis, graphing and statistical functions. Unlimited use software license for Windows XP, Pentium IV (2GHz) or higher PC.Rotor Gene 3000 Real-Time PCR Machine Features:. Unique centrifugal rotary format. Excitation Source: LED high power diodes, 470nm, 530nm, 585nm and 625nm. Detection Filters: 510nm (FAM), 555nm (JOE), 610nm (ROX) and 660nm high-pass (Cy5). Holds 36 x0.2ml tubes or 72 x 0.1ml strip tubes.

Software: Build-in extensive analysis, graphing and statistical functions.Use of the machines in the Molecular Biology Facility. The Rotor-Gene machines are in high demand therefore there is an (external website). Plan ahead, book time slots and stick to them. Users are responsible for supplying all their own plastic ware and reagents.

0.1ml tubes and Gene-Disc 100 are produced by Corbett especially for the Rotor-Gene. Save files in your own folder in the 'Rotor Gene Run File' folder in the local disk (C:). The computer is also linked to the network for easy transfer of your data to the server.Tips and addition information. The Rotor-Gene machines support reaction volumes from 5 µl to 100 µl (rotor and protocol dependent). Typical reaction volume is 20µl. The use of a normalisation dye, such as ROX reference dye, is optional on the Rotor-Gene. Many companies supply master mixes for real-time PCR which can decrease the chance of pipetting errors in this highly sensitive method.

Rotor Gene 6000 Software Programs For Windows 10

If you would like to learn more about the real-time PCR experimental design and data analysis, please contact Dr. Donna Lai, the Molecular Biology Officer.